Anti-immunoglobulin antisera used in an ELISA to detect antibodies in barramundi Lates calcarifer to Cryptocaryon irritans

Anti-immunoglobulin antisera used in an ELISA to detect antibodies in barramundi Lates calcarifer to Cryptocaryon irritans.

Immunoglobulins (Ig) in serum from barramundi vaccinated with bovine serum albumin (BSA) were purified by ammonium sulphate precipitation and affinity chromatography using BSA as the ligand. The BSA-binding activity of eluted putative Ig fractions was assessed by enzyme-linked immunosorbent assay (ELISA) before being pooled and characterised by sodium dodecyl sulphate polyacrylamide gel electro...

Defining essential fatty acid deficiency in juvenile barramundi Lates calcarifer

Barramundi (Lates calcarifer), a catadromous teleost of significant and growing commercial importance, are reported to have limited fatty acid bioconversion capability, typical of marine species, and therefore require preformed long-chain polyunsaturated fatty acids (LC-PUFA) as dietary essential fatty acid (EFA). In this study, the response of juvenile barramundi (47.0 g/fish initial weight) f...

A microsatellite linkage map of Barramundi, Lates calcarifer.

Barramundi (Lates calcarifer) is an important farmed marine food fish species. Its compact genome (approximately 700 Mb) is among the smallest genomes of food fish species. We established a first-generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progeny. A total of 240 microsatellite markers were mapped into 24 linkage g...

Modelling Size-Dependent Cannibalism in Barramundi Lates calcarifer: Cannibalistic Polyphenism and Its Implication to Aquaculture

This study quantified size-dependent cannibalism in barramundi Lates calcarifer through coupling a range of prey-predator pairs in a different range of fish sizes. Predictive models were developed using morphological traits with the alterative assumption of cannibalistic polyphenism. Predictive models were validated with the data from trials where cannibals were challenged with progressing incr...

The use of biomarkers in barramundi (Lates calcarifer) to monitor contaminants in estuaries of Tropical North Queensland